TABLE 5.
Effect of a NOS inhibitor on the trypanocidal activation of myocardial cellsa
| IFN-γ (5 U/ml) | l-NIL (0.5 mm) | No. of parasites/100 cells (% infected cells)
|
|
|---|---|---|---|
| WT | TNFRp55−/− | ||
| − | − | 86 ± 24 (9.0 ± 3.0) | 72 ± 21 (7.4 ± 2.6) |
| + | − | 26 ± 3b (7.6 ± 0.4) | 12 ± 1b (5.2 ± 2.1) |
| + | + | 69 ± 14 (8.4 ± 2.7) | 51 ± 18 (6.6 ± 0.6) |
a
Recombinant murine IFN-γ was or was not added to myocardial cells in the presence or absence of l-NIL. Six hours after infection, free parasites were washed and IFN-γ and l-NIL were added again. Mean numbers of infected cells and numbers of parasites per 100 cells ± standard errors of the means were recorded from triplicate wells 72 h after infection. −, absence; +, presence.
b
Difference from the nontreated control value is significant (Mann-Whitney-U Wilcoxon test).