REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) 1:1000	Cell Signaling Technology	#9101; RRID:AB_331646
Phospho-HSL (Ser563) 1:1000	Cell Signaling Technology	#4139; RRID:AB_2135495
Phospho-Stat3 (Tyr705) (D3A7) XP® 1:1000	Cell Signaling Technology	#9145; RRID:AB_2491009
Itgam 1:500	Atlas Antibodies	AMAb90911; RRID:AB_2665722
Biological samples
Primary human myoblasts	Previous works from the group	N/A
Human in vitro differentiated adipocytes	Gao et al., 201759	N/A
Human subcutaneous leg fat biopsies	This paper	N/A
Human plasma samples	This paper	N/A
Human subcutaneous abdominal fat biopsies	Fabre et al.20	N/A
Chemicals, peptides, and recombinant proteins
Oncostatin-M	Bio-Techne LTD	295-OM-050
GDF15	Bio-Techne LTD	9279-GD-050
SFRP4	Bio-Techne LTD	1827-SF-025/CF
MRXA5	Nordic Biosite	LS-G26239-10
Isoproterenol	Merck	I5627
Clenbuterol	Merck	C5423-10MG
Forskolin	Merck	F6886-10MG
IBMX	Merck	I5879-250MG
Insulin	Novo Nordisk	Actrapid
Dexamethasone	Merck	D4902-1G
Rosiglitazone	GlaxoSmithKline	BRL49653-C
apo-transferrin	BBI solution	T100-5
Recombinant Human IL4	PeProTech	200–04
Recombinant Human IL13	PeProTech	200–13
Recombinant Human IFNg	PeProTech	300–02
Phorbol 12-myristate 13-acetate (PMA)	Merck/Sigma-Aldrich	P1585
Lipopolysaccharides	Merck/Sigma-Aldrich	L9641
Hoechst 33342	Abcam	Ab228551
Lectin	Vector Laboratories	DL-1048-1
TRIzol	Thermo Fisher Scientific	15596018
Triton X-100	Sigma-Aldrich	X100-100ML
Tween 20	Sigma-Aldrich	P1379-1L
DAKO Fluorescence mounting media	Agilent Technologies	S302380-2
Protein Block	Agilent Technologies	X090930-2
Protease Inhibitor Cocktail	CALBIOCHEM	539131–10
ECL reagent	GE Healthcare	RPN2106
Critical commercial assays
TruSeq Stranded Total RNA with Ribo-Zero Gold	Illumina	20020598
Rneasy lipid tissue kit	Qiagen	Cat#74804
Agilent RNA 6000 nano kit	Agilent	5067–1511
Qubit dsDNA HS assay kit	Invitrogen	Q32851
Agilent High Sensitivity DNA chip	Agilent	5067–4626
Human Oncostatin M/OSM ELISA Kit	Abcam	ab215543
EZNA total RNA kit I	Omega Bio-tek	R6834-01
Pierce BCA Protein Assay Kit	ThermoFisher	Cat#23227
Multiplex Fluorescent detection reagents v2	ACDBio	323110
Free glycerol Reagent	Sigma-Aldrich	#F6428
Amplex Ultrared Reagent	Invitrogen	#A36006
Deposited data
Human adipose tissue in response to exercise (RNAseq)	This paper	GEO: GSE198922
Experimental models: Cell lines
Thp1 monocytes cell line	ATCC	TIB-202
Oligonucleotides
TaqMan probe human OSM	Applied Biosystems	Hs00171165_m1
TaqMan probe human GDF15	Applied Biosystems	Hs00171132_m1
TaqMan probe human LIF	Applied Biosystems	Hs01055668_m1
TaqMan probe human OSMR	Applied Biosystems	Hs00384276_m1
TaqMan probe human LIFR	Applied Biosystems	Hs01123581_m1
TaqMan probe human IL6ST	Applied Biosystems	Hs00174360_m1
TaqMan probe human IL6	Applied Biosystems	Hs00174131_m1
TaqMan probe human CXCL2	Applied Biosystems	Hs00601975_m1
RNAscope™ Probe- Hs-OSM	ACDBio	456381
RNAscope™ Probe- Hs-CD68-C2	ACDBio	560591-C2
Software and algorithms
GraphPad Prism 9.0	GraphPad Software	N/A
R v4.3.0	R	N/A
Zen Pro	Zeiss	N/A
Quantity One 1-D Analysis Software	Bio-Rad	N/A
Python	Python	https://www.python.org/
Zeiss Zen Pro Software	Zeiss	N/A
Standard inverted multichannel Carl Zeiss Axio Obeserver Micropscope (Leica camera 20x objective)	Zeiss	N/A
Fiji		https://fiji.sc
QuPath Image Processing Software	QuPath	https://qupath.github.io/
QuantStudio 7 Real-Time PCR	Thermo Fisher Scientific	N/A
BioNavigator®	PamGene	N/A
Cytoscape	Cytoscape Team	https://cytoscape.org/
NIS Elements Software	Nikon	N/A
Nikon inverted TiE microscope	Nikon	N/A