Figure 6.

Aberrant platelet transcriptomes coincident with PEM in ME/CFS

(A) Schema depicting paired analysis (intraindividual expression) of gene expression altered by strenuous exercise in ME/CFS patients compared to controls.

(B) Number of significantly enriched gene sets across clusters (x axis) in a paired analysis comparing case and control ΔRNA measurements with GSEA.

(C) GSEA results for significantly enriched gene sets related to platelet function. GSEA analyses included the comparison of paired ΔRNA measurements between cases and controls (dark purple for scRNA-seq and pink for RNA-seq), as well as comparing group averages at BL (red for scRNA-seq and blue for RNA-seq) and PC (none detected).

(D) Enrichment plot depicting representative significantly enriched gene sets related to platelet function and translation using the intraindividual paired analysis for platelets (cluster 19) from scRNA-seq.

(E) Heatmap of leading-edge genes taken from the gene set “GOBP regulation of platelet activation” between 3 healthy controls (HC) and 3 ME/CFS cases (ME) at BL and PC; expression values row-normalized.

(F) Enrichment plot depicting the same gene sets shown in (D) using the intraindividual paired analysis of bulk RNA-seq of platelet-derived plasma particles.

(B–D) Data from a cohort of 28 healthy controls and 30 ME/CFS cases. (E and F) Data from 3 healthy controls and 3 ME/CFS cases (all females) at both time points.