Cryoablation only (Cryo) and cowpea mosaic virus–treated only (CPMV), alone or in combination, significantly retarded treated tumor growth. Fold change in tumor size at 2 weeks after treatment initiation: the CPMV, Cryo, and cryoablation plus CPMV–treated (Cryo + CPMV) groups demonstrated significantly slower growth compared with that in the control group (black). Cryo + CPMV resulted in marked reduction of tumor growth compared with that in the control group (1.6 ± 0.9 vs 6.3 ± 0.5, P < .0001). This change did not reach significance compared with CPMV or Cryo alone. The fold change growth of CPMV was 3.2 ± 2.6 (P = .01), and that of Cryo was 2.5 ± 2.4 (P = .002). The asterisks indicate statistical significance: *P < .05, **P < .01, and ****P < .0001.