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Summary of results
| Procedurea | Formation of rings | Gold particles on membrane |
|---|---|---|
| BLY (RT) | + | |
| BLY (0°C) | − | |
| BLY (0°C) + FA + IM | − | + |
| BLY (0°C) + wash + 37°C | + | |
| BLY (0°C) + GA + 37°C | − | |
| GA + BLY (RT) | + | |
| BLY + 10 mM ZnSO4 (RT) | ||
| Alone | − | |
| + FA + IM | − | + |
RT, incubation at room temperature; 0°C, incubation at 0°C; FA, formaldehyde; IM, immunoelectron microscopy; GA, glutaraldehyde; 37°C, incubation at 37°C.
