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. 2023 Dec 12;626(7997):207–211. doi: 10.1038/s41586-023-06905-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 9 — A-E) Left panels show the lacZ intensity (green) as a marker for the enhancer activity pattern of the respective candidate sequence (labeled on the left). Right panels show the intensity of both the lacZ reporter gene driven by the synthetic sequence (green) and the corresponding endogenous marker gene (pink) for the respective tissue (elav (CNS), wg (epidermis), GATAe (gut), Mef2 (muscle) and tll (brain)). Synthetic enhancers are labeled as correct tissue expression, incorrect tissue expression and inactive. For each sequence, one representative embryo is shown from the total 200–300 double FISH-stained embryos. Scale bar, 100 μm. See Table S2 for more details.