Extended Data Fig. 8. EMSA shows stronger binding of the human HOXA13 and HOXD13 DBDs to the Dutch 2 variant relative to the WT sequence.

a, WT probe sequence can bind to the human HOXA13 DBD to generate a band, the intensity of which decreases in the presence of the non-biotin competitor probe. The Dutch 2 variant binds to HOXA13 more strongly than does the WT allele. A single-bp change that ablates HOXA13 binding shows no binding. b, EMSA with the same WT and Dutch 2 probe sequences as in a, performed with the human HOXD13 DBD. The Dutch 2 variant binds to HOXD13 more strongly than does the WT allele. EMSAs were performed independently twice and both replicates show similar results. For gel source data, see Supplementary Fig. 1.