Extended Data Fig. 4. Comparison of sub-mitochondrial protein population in the mitoplast sample and APEX-labeled proteome of MAX-Tg mice (whole body expressed MAX-Tg mice and Myf5-Cre; LSL-MAX-Tg mice).
(a) Validation of mitoplast with western blot using antibodies for sub-mitochondrial marker proteins. Representative images from three independent experiments. (b) Comparison of identified proteins in the mitoplast sample from TA muscle (WT mice), APEX-labeled proteins in TA muscle of MAX-Tg sample and APEX-labeled proteins in TA muscle of Myf5-Cre; MAX-Tg mice (c) Sub-mitochondrial protein population of mitochondria-annotated proteins in the mitoplast, APEX-labeled proteins of MAX-Tg (MAX), APEX-labeled proteins of Myf5-Cre; LSL-MAX-Tg (Myf5-MAX) in TA muscle samples. Sub-mitochondrial protein annotations were generated using Mitocarta3.0 (see Supplementary Data 5 for detailed information).