Table 1.
Successful virus isolation and titration from positive quantitative Reverse-Transcription PCR samples collected from adult filovirus-inoculated AFBs
| EBOV | MARV | |||||||
|---|---|---|---|---|---|---|---|---|
| 5 dpi | 10 dpi | 5 dpi | 10 dpi | |||||
| TISSUE | PCR; | (VI) | PCR; | (VI) | PCR; | (VI) | PCR; | (VI) |
| Lymph Node | 3/3; | -- | 2/2; | (0/1) | 1/3; | (0/1) | 0/2; | -- |
| Uterus/Placenta | 1/1; | (1/1) | 1/1; | (1/1) | 1/1; | (1/1) | ||
| Blood | 3/3; | (1/1) | 1/2; | -- | ||||
| Spleen | 3/3; | (1/2) | 2/2; | -- | ||||
| Colon | 3/3; | (1/1) | 1/2; | -- | ||||
| Kidneys | 3/3; | (1/1) | 1/2; | -- | ||||
| Bladder | 3/3; | (2/2) | 1/2; | -- | ||||
| Testes | 2/2; | (1/1) | 0/1; | -- | ||||
| Brain | 3/3; | (1/1) | 0/2; | -- | RESTV | |||
| Salivary Gland | 2/3; | (0/2) | 1/2; | -- | ||||
| Oral Swab | 3/3; | (1/2) | 0/2; | -- | 5 dpi | 10 dpi | ||
| Rectal Swab | 3/3; | (1/2) | 1/2; | -- | PCR; | (VI) | PCR; | (VI) |
| Spleen | 3/3; | (1/2) | 2/2; | -- | 2/3; | (0/1) | 0/3; | -- |
| Gallbladder | 1/1; | (1/1) | 2/2; | (1/1) | 1/3; | (0/1) | 2/3; | -- |
| Stomach | 2/3; | (0/1) | 0/3; | -- | ||||
| Skin Inoculation | 3/3; | (1/2) | 0/3; | -- | ||||
| Pooled faeces* | 3 dpi (+/+); 5 dpi (+/−); 10 dpi (+/−) | |||||||
| Pooled urine* | 10 dpi (+/−) | |||||||
PCR, polymerase chain reaction; VI, Virus isolation; dpi, days post inoculation.
* Only dpi collection with attempted virus isolation shown: (PCR/VI). (+) Positive; (-) Negative; (--) Virus isolation not attempted: virus RNA loads below the cut-off.