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Determination of the IgG subclass of highly immunogenic H. pylori LPS-specific antibodies in human sera. LPS derived from strain GU2 was coated onto a microplate. Human serum (1,000-fold dilution) was applied to the plate. Subsequently, human IgG subclass-specific murine monoclonal antibodies, peroxidase-conjugated goat anti-mouse IgG antibodies, and TMB substrate solution were applied. Binding activity was expressed as absorbance at 450 nm.
