Fig. 5.

Effect of prenatal dexamethasone treatment and acute stress in adulthood on the level of FNDC5 protein (A), its cleaved form, irisin (B), and BDNF (C) in the frontal cortex and hippocampus (D, E, and F, respectively) of male rats; the level of the two former proteins was measured using ELISA and expressed in ng/mg of protein, and the latter was estimated by Western blot and expressed as a fold change; bar graphs represent the mean ± SEM. Statistics: two-way ANOVA; n = 8–10; in the case of irisin, stress main effect was significant at F_1,32 = 11.096; p = 0.002 in the frontal cortex and F_1,29 = 7.462; p = 0.011 in the hippocampus