Fig. 6.

Effect of prenatal dexamethasone treatment and acute stress in adulthood on the level of IGF-1 (A) and its receptor phospho-IGF-1R (B) and total form IGF-1R (C) in the frontal cortex and hippocampus (D, E, and F, respectively) of male rats; the level of IGF-1 protein was assessed using ELISA and expressed in ng/mg of protein, while pIGF-1R and IGF-1R were estimated with Western blot and expressed as a fold change; bar graphs represent the mean ± SEM. Statistics: two-way ANOVA; n = 9–10; in the case of IGF-1 in the hippocampus, DEX main effect was significant at F_1,32 = 4.722; p = 0.037, while stress effect in the case of pIGF-1R at F_1,36 = 7.272; p = 0.011 in the frontal cortex and F_1,35 = 6.364; p = 0.016 in the hippocampus