Figure 2.

Both (A) H524 and (B) H82 SCLC cells were treated with cisplatin (CT; 0.5 µM) for 72 h and then irradiated at 4 Gy (RT). After irradiation, both cell groups (1 × 10⁴ cells/well) were cultured for 24 h and mRNA expression levels of innate immune activation markers in both SCLC cell lines were determined by quantitative real-time PCR (see Supplementary Material). Normalized expression data are represented as mean ± s.e.m. derived from three technical replicates calculated using the comparative method of 2-^(ΔΔCt) (reference gene 18S). One-tailed unpaired Student’s t-test with 95% confidence interval (CI), where ****P<0.0005, ***P<0.005, and **P< 0.05.