FIGURE 3.
Effect of hypoxia on SSC quiescence and differentiation (A) KI67 labeling in PLZF+ SSCs population under hypoxic conditions (3.5%, 1% and 0.1%), 21% O2 and growth factor/serum-deprived conditions (SS). (B) Analysis of the percentage of G0 cells (KI67neg cells) after 4 days under hypoxic conditions or growth factor/serum-deprived conditions (SS) (n = 3). (C) Observation of the differentiation of SSCs by KIT receptor expression in PLZF+ cells under hypoxic, differentiation (ATRA) or growth factor/serum-deprived conditions after 4 days of culture. (D) Analysis of the ratio of the number of KIT− cells to KIT+ cells (n = 3).