FIG. 2.

Demonstration of elevated Hsp synthesis in McCoy cell cultures. Western immunoblotting of the serum of a patient from whom an S. aureus Endo-2 clinical isolate was obtained against bacterial cell lysate proteins. Lanes: 1, S. aureus maintained at 30°C; 2, S. aureus maintained at 37°C; 3, S. aureus shifted from 30 to 37°C; 4, cycloheximide-treated McCoy cells; 5, heat-killed bacteria added to McCoy cells; 6 to 8, S. aureus grown at 37°C and added to McCoy cells at 37°C (1, 2, and 3 h postinfection, respectively); 9 to 11, S. aureus grown at 30°C and added to McCoy cells at 37°C (1, 2, and 3 h postinfection, respectively).