Fig. 3. Transcriptional alterations in specific neuronal cell types in human striatum associated with OUD.

a Expression heatmap of z-normalized pseudobulk gene expression, where the rows are differentially expressed genes (DEGs) and the columns are the biological replicates labeled by cell type, diagnosis, and sex of each individual. Genes that are represented by the enriched pathways in (c, d) are labeled along the right margins. b A heatmap plotting the t-statistic of differentially activated transcription factor gene regulatory networks across neuronal subtypes in opioid use disorder (OUD). c, d Network plot of significant clusters and enriched pathways, where each point is a significantly enriched pathway in a neuronal cell type and lines represent the proportion of shared genes between two pathways. Each point is colored by the normalized enrichment score (NES), indicating whether a pathway is enriched in upregulated or downregulated genes. The color outlines represent the unique clusters of interconnected pathways by shared genes and the nearby text labels the unique cluster number and briefly summarizes which cell types and pathways are represented. e Cluster maps of select transcription factor gene regulatory networks. Each transcription factor or gene is colored to denote the direction and significance of differential expression in neuronal subtypes in OUD. Source data are provided as a Source Data file. Source data are provided as a Source Data file.