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Heterogeneous expression of CD14 mRNA by CD14high and CD14low HGF. CD14high HGF (lane 1, donor MH; lane 2, donor SM) and CD14low HGF (lane 3, donor KT; lane 4, donor NK) from confluent cultures were collected by trypsinization. RNA was extracted from the cells, and its cDNA was prepared and analyzed by RT-PCR. The results are representative of three different experiments.
