FIG. 4.

The release of sCD14 by CD14^high HGF was augmented by IFN-γ and LPS. (A) Confluent CD14^high HGF (donor MH) and CD14^low HGF (donor KT) were incubated with medium alone, with IFN-γ (200 IU/ml), or with LPS (100 ng/ml). Supernatants were collected at days (d) 1, 2, and 3 and analyzed for the presence of sCD14 by ELISA. Results are the means ± standard deviations of duplicate determinations from one representative experiment. ∗, P < 0.05 versus medium alone. (B) Confluent CD14^high HGF (donor MH) were incubated with IFN-γ (200 IU/ml) for the times indicated and were collected by trypsinization. RNA was extracted from the cells, and its cDNA was prepared and analyzed by RT-PCR. The results are representative of three different experiments.