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SDS-PAGE of Manfredo CE inhibitory fractions following anion-exchange chromatography and gel filtration chromatography. Lanes: 1, phosphorylase b (97 kDa); 2, total CE (0.5 μg); 21–26, fractions 21 to 26 after resolution of CE by anion-exchange chromatography and gel filtration chromatography (see Fig. 3); 9, cytochrome c (12 kDa); 10, chymotrypsinogen A (25 kDa). The gel was silver stained by the method of Hochstrasser et al. (19).
