TABLE 4.
Comparison of AD levels present in different streptococcal CEa
| Strain or prepn | AD activity (μM citrulline produced/h/μg of protein) | ID50 (μg/ml) |
|---|---|---|
| Strains | ||
| M5 Smith | 40.3 | 1.0 |
| M5 NCTC 8193 | 30.7 | 1.2 |
| M80 | 39.2 | 1.5 |
| M14 NCTC | 11.4 | 1.2 |
| M5 Manfredo | 16.1 | 1.2 |
| M5 R91/1974 | 36.7 | 1.6 |
| PT2841 | 17.3 | 1.9 |
| PT3875 | 15.6 | 2.4 |
| M27 NCTC 8328 | 14.1 | 2.5 |
| M2 NCTC 8322 44/R64 | 8.6 | 3.7 |
| PT2110 | 4.1 | 7.0 |
| M15 NCTC | 2.7 | >10 |
| Manfredo FPLC-purified fraction | 84.7 | ND |
a
Results are representative of those obtained from two separate measurements. AD activity was assayed as described in Materials and Methods. ID50, CE concentration that causes 50% inhibition of proliferation of PBMC stimulated with PHA in the presence of a noninhibitory concentration of CE (0.625 μg/ml). ND, not determined.