Figure 3.

HSV-1 vhs protein expression modulates loss of total and surface MR1

(A) ARPE-19 MR1-GFP cells were infected with RAd-Ctrl adenovirus (black or dotted line) or RAd-Ctrl modified to encode HSV-1 vhs (RAd-vhs, green), MOI 100. Cells were left untreated (nil) or treated with Ac-6-FP (5 μM) for 4 h prior to harvesting at 44 h p.i. (post). Cells were stained for surface MR1, MHC-I, or matching isotype control (gray) and analyzed by flow cytometry. Fold change in MFI of live infected cells relative to RAd-Ctrl with matching ligand treatment is represented as mean ± SEM. Statistical significance was calculated by paired Student’s t test, ∗p < 0.05, ∗∗∗∗p < 0.0001. Analysis of 4 independent experiments.

(B) ARPE-19 MR1 cells were infected with RAd-Ctrl adenovirus or RAd-vhs or mock infected. Cells were harvested at 44 h p.i. and lysates were separated by gel electrophoresis before immunoblotting for MR1, MHC-I, and GAPDH.