Table 3.
Receptor binding affinities of human insulin, human IGF-1 and VILPs to human IGF1R. Binding affinity is reported by the equilibrium dissociation constant (Kd). The Kd values were obtained from at least three independent measurements (indicated as n).
| Ligand | IGF1R |
|---|---|
| Kd [nM] ± S.D. (n) | |
| Human IGF-1 | 0.24 ± 0.13 (3)# |
| 0.30 ± 0.06 (5)$ | |
| Human insulin | 293 ± 101 (3)# |
| scMFRV | 2.85 ± 0.09 (3)# |
| dcMFRV | 16.3 ± 0.6 (3)$ |
| scLCDV-Sa | 60.8 ± 18.3 (3)# |
| dcLCDV-Sa | 639 ± 453 (4)# |
The Kds of ligands were determined in two independent measumerements (# and $).