Fig. 1.

Differences in frequencies of tumor-infiltrating leukocytes in patients with OPSCC and CESC. (A) The figure shows the abundance of immune cells estimated by the CIBERSORT-ABS tool from TCGA datasets. (B) Columns represent mean (+ standard error of mean, SEM) densities of immune cells in tumor stroma and tumor epithelium of immunohistochemically stained FFPE sections of HPV+ OPSCC and CESC samples. (C) Violin plot shows the distribution of TLS densities in OPSCC and CESC samples. Bold lines represent medians, and thin lines represent quartiles. (D) Pie charts show the proportions of patients with cold, excluded, and hot tumor microenvironment types. The stratification is based on CD8+ Tcell density in the tumor stroma and tumor epithelium. (E) Scatterplots show the correlation between densities of myeloid cells and CD8+ T cells in the tumor epithelium of combined cohorts of OPSCC and CESC patients. * p < 0.05 (Mann–Whitney U test).