| Reagent or resource | Final concentration | Amount |
|---|---|---|
| 4% Paraformaldehyde | ||
| Paraformaldehyde Powder | 4% | 20 g |
| 5 N NaOH | 0.02% | 100 μL |
| Milli-Q water | N/A | 450 mL |
| Immunofluorescence Blocking solution | ||
| BSA | 3% (m/v) | 3 g |
| Tween-20 | 0.05% (v/v) | 100 μL |
| Make fresh and store at 4°C for up to a week | ||
| Primary Antibody Staining solution (Primary antibodies were dissolved in PBS-T (0.05%)) | ||
| Anti-PDHA1 mouse monoclonal | 1:400 | 1.25 μL |
| Tom20 Polyclonal | 1:200 | 2.5 μL |
| DAPI | 1:1000 | 0.5 μL |
| Make immediately prior to staining | ||
| Secondary Antibody Staining solution (Secondary antibodies were dissolved in 1× PBS) | ||
| Goat anti-Mouse IgG (H + L) | 1:200 | 2.5 μL |
| Goat anti-Rabbit IgG (H + L) | 1:200 | 2.5 μL |
| Make immediately prior to staining | ||
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