REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-PDHA1 mouse monoclonal	Abcam	Cat# ab110330
TOM20 polyclonal	Proteintech	Cat# 11802-1-AP
Goat anti-mouse IgG (H + L)	Thermo Fisher Scientific	Cat# A-11004
Goat anti-rabbit IgG (H + L)	Thermo Fisher Scientific	Cat# A-11034
DAPI	Thermo Fisher Scientific	Cat# 62248
Chemicals, peptides, and recombinant proteins
BSA	Sigma	Cat# A4503-100G
Sucrose	Sigma	Cat# S0389-500G
Paraformaldehyde	Sigma	Cat# P6148-500G
Tween 20	Sigma	Cat# P9416-100ML
1× PBS	Quality Biological	Cat# 114-058-101CS
Ethanol	Fisher Scientific	Cat# FSA4094
Xylene	Sigma	Cat# 534056-4L
EDTA	Sigma	Cat# E9884-500G
Experimental models: Organisms/strains
Mouse: C57BL/6	In-house	8–10 weeks old male mice
Software and algorithms
NIS-Elements
ImageJ	NIH	https://imagej.nih.gov/ij/
GraphPad Prism
Other
Pap pen	Abcam	Cat# ab2601
Mounting media	Sigma	Cat# 06522-100 ML
Coverslip (24 × 50 mm)	Fisher Scientific	Cat# 12544E
Whatman filter paper	GE Healthcare Life Sciences	Cat# 1001-240
Plastic case	Fisher Scientific	Cat# NC0731404