Table 1. Primers used for the respiratory syncytial virus RSVAB-WGS and RSVAB-GF sequencing method.
Primer | Sequence (5’–3’) |
---|---|
Mix 1 RSVAB WGS | |
RSVCombinitial | ACGCGAAAAAATGCGTACWACA |
RSVWGS4R | CATGWTGWYTTATTTGCCCC |
RSVWGS2F | CACTWACAATATGGGTGCC |
RSVWGS1R | TCCATKGTTATTTGCCCC |
RSVWGS3.2F | ACATGGAAAGAYATYAGCC |
RSVWGS2R | CRTTYCTTAARGTRGGCC |
RSVWGS3.2R | TTGCATCTGTAGCAGGAATGG |
RSVCombending | ACGAGAAAAAAAGTGTCAAAAACTAA |
Mix 2 RSVAB WGS | |
RSVCombinitial | ACGCGAAAAAATGCGTACWACA |
RSVWGS1R | TCCATKGTTATTTGCCCC |
RSVWGS2F | CACTWACAATATGGGTGCC |
RSVWGS8R | TCMAWYTCWGCAGCTCC |
RSVWGS5R | CAAACATTTAATCTRCTAAGGC |
RSVWGS6F | TTATAYAGATATCAYATGGGTGG |
RSVWGS6R | CCCTCTCCCCAATCTTTTTC |
RSVCombending | ACGAGAAAAAAAGTGTCAAAAACTAA |
Mix RSVAB-GF | |
OG1-21 | GGGGCAAATGCAACCATGTCC |
RSVGF-R | TTCGYGACATATTTGCCCC |
The primers were organised by their position in the genome and the amplification mix in which they were employed. Primers RSVCombinitial, RSVWGS1R, RSVWGS2F and RSVCombending were used in both mixes.