Table 2. Respiratory syncytial virus reference controls used to standardise the RSVAB-WGS and RSVAB-GF sequencing methods.
| Strain | ATCC nomenclature | Cq value | Number of copies/mL | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Dilution of control virus | 10-1 | 10-2 | 10-3 | 10-4 | 10-1 | 10-2 | 10-3 | 10-4 | |
| RSV A Long | VR-26 | 15.12 | 18.88 | 22.09 | 25.57 | 2 × 104 | 2 × 103 | 2 × 102 | 2 × 101 |
| RSV B CH-18537 | VR-1580 | 16.21 | 19.5 | 22.8 | 26.2 | 2 × 104 | 2 × 103 | 2 × 102 | NA |
ATCC: American type culture collection; Cq: quantification cycle; NA: not applicable.
The standardisation process included quantifying the dilutions of each control virus based on the Cq value obtained in the RT-PCR used for viral detection and subtyping. Subsequently, we calculated the number of copies of viral nucleic acids in each dilution of the control viruses.