Table 1.

Hormone measurements and analytical methods of serum hormones measured.

Analyte	Assay name	Manufacturer	Instrument	CV%			Reportable range	Reference value	Specimen
				Low contr	Med. contr	High contr			Type	Storage
TSH	TSH	Roche	Elecsys	11.1	1.3	2.2	0.005–100 mIU/L	0.270–4.20 mIU/L	Serum	+2–8°C
fT4	FT4 III	Roche	Elecsys	4.3	1.5	2.6	0.5–100 pmol/L	12–22 pmol/L	Serum	+2–8°C
Prolactin	Prolactin II	Roche	Elecsys	1.5	3.0	2.4	0.0470–470 μg/L	Female (not pregnant) 4.79–23.3 μg/L	Serum	+2–8°C
Cortisol	Cortisol II	Roche	Elecsys	5.4	1.5	1.6	1.5–1750 nmol/L	Morning (06:10 h): 133–537 nmol/L, afternoon (16:08 h): 68.2–327 nmol/L	Serum	+2–8°C
IGF1	IGF-I	Siemens	Immulite 2000	6.3	3.1	2.5	15–1000 μg/L	Manufacturer’s age-dependent reference rangea	Serum	+2–8°C or −20°C
ACTH	ACTH	Roche	Elecsys	2.7	0.6	0.7	1.0–2000 ng/L	7.2–63.3 ng/L	Plasma	+2–8°C
GH	Growth hormone	Siemens	Immulite 2000	6.5	5.5	6.6	0.05–40 ng/mL	Females: up to 8 ng/mL	Serum	+2–8°C
Anti-TPO	EliA anti–TPO	Thermo Fisher	Phadia™ 250	–	–	–	–	<25 IU/mL = negative, 25–35 IU/mL = equivocal, >35 IU/mL = positive	Serum	+2–8°C

^aImmulite product booklet, IGF-1, Immulite 2000 IGF-1 (PIL2KIGF-4, 2018–07-02).

ACTH, adrenocorticotropic hormone; Anti-TPO, anti-thyroid peroxidase antibodies; CV, coefficient of variation; fT4, free thyroxine; GH, growth hormone; High contr, high control; IGF1, insulin-like growth factor 1; Low contr, low control; Med. contr, median control; TSH, thyroid-stimulating hormone.