Fig. 10.

Live and Dead cells differentiation by Acridine Orange/Ethidium Bromide (AO/EB) staining

Under the TRITC filter, a bright orange color nucleus with a contrasting red color cytoplasm was seen in the picture due to the loss of cell integrity in the dead cells. Live cells exhibit green fluorescence when seen through a FITC filter because their cell membranes are intact. (A) Untreated A549 cells observed in FITC filter (B) Untreated A549 cells observed in TRITC filter (C) DS@AgPVP NPs treated A549 cell observed in FITC filter (D) DS@AgPVP NPs treated A549 cell observed in TRITC filter (E) AC@AgPVP NPs treated A549 cell observed in FITC filter (F) AC@AgPVP NPs treated A549 cell observed in TRITC filter (G) AgPVP NPs treated A549 cell observed in FITC filter (H) AgPVP NPs treated A549 cell observed in TRITC filter. Arrows indicate the changes due to the treatment of Nanoparticles compared to the untreated cells. The scale bar indicates 10 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)