Fig. 2.

Protective effect of WSY6 on H₂O₂-induced cytotoxicity. (A) PIG1 cells were treated with various concentrations of WSY6 for 24 h and cell viability was determined by MTS assay. (B) PIG1 cells were pretreated with or without various concentrations of EGCG or WSY6 for 1 h. Then, cells were treated with 1 mM H₂O₂ for 1 h. After treatment, cells were incubated in medium for 24 h and the cell viability was determined by MTS assay. (C) The effect of EGCG or WSY6 on H₂O₂-induced morphological change in PIG1 cells. Scale bar is 100 μm. (D) Determination of the viability of PIG1 cells by an LDH release assay. Dates were presented as the mean ± SD. (n = 3/group, Data were compared using One-Way ANOVA, ns: no significant difference, **p < 0.01, ***p < 0.001, ****p < 0.0001).