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Spike recovery and parallelism
Four aliquots of each two plasma samples with different TDP-43 concentrations were spiked with 0, 1, 5, and 10 pg of TDP-43 standard in 360 μL of solution. These 8 (4 × 2) aliquots were analyzed in duplicate. We made two spike recovery curves starting from nonspiked solution to 10 pg-spiked solution to evaluate parallelism. Recovery rates (%Recovery) were calculated at each concentration.
