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Dilution linearity
The high-concentration spiked sample prepared in the spike recovery tests (Fig. 2) was serially ( × 2, × 4, × 8, × 16, and × 32) diluted with the sample diluent until the theoretical concentration reached the LLOQ. The TDP-43 levels in the serially diluted samples were measured in duplicate and compensated for the dilution factor. The %Recovery for the dilution-corrected concentration was calculated at each dilution.
