Fig. 2.
Characterization of the EGFP-NF-L reporter PC12 cell line: PC12-B. (A) Scheme of the monoallelic knock-in of EGFP at the Neurofilament-L locus. Cell line generation was performed by targeting the locus of interest via CRISPR/Cas9. (B) Fluorescence microscopy of PC12-B cells after 6 days of 100 μl/ml Cerebrolysin® treatment. (C) Flow cytometry analysis representing EGFP-Neurofilament-L expression of PC12-B cells after treatment with 50 ng/ml NGF or 100 μl/ml Cerebrolysin® for 4 days. Untreated cells serve as a control. One representative out of 25 independent experiments. (D) Statistical analysis of MFI of EGFP from PC12-B cells after treatment with 50 ng/ml NGF or 100 μl/ml Cerebrolysin® for 4 days. Untreated cells serve as a control. Boxes represent 25th to 75th percentile (including median), whiskers represent min to max. Control n = 31, NGF n = 28, Cerebrolysin® n = 25. ****p < 0.0001. Kruskal-Wallis test followed by multiple comparison of each condition against control, correction for multiple comparison using Dunn's test.