Figure 1.

Establishment of a mouse model of diet-induced obesity. (a) Mice were fed with either high fat (45% energy by fat) or a standard, low fat (10% energy by fat) diet for at least 29 weeks to establish obese or control mice prior to sensitization to peanut (PN) via inhalation. Mice were weighed weekly and their weights are reported in grams. Data represents a mean ± SEM of 24 mice per a diet group. (b) Percent increase in weight of mice fed HFD as compared to mice fed LFD. Data are shown starting 3 weeks after diet commenced and is recorded weekly through week 31. (c) Leptin plasma levels were measured after 8 and 15 weeks via ELISA. Data is the mean ± SEM of 9 of the 24 mice per diet group randomly selected to test for leptin levels in plasma. For both (a) and (c), * p < 0.05 compared to control mice fed LFD, unless indicated otherwise.