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. 2024 Jan 9;27(2):108843. doi: 10.1016/j.isci.2024.108843

Figure 5.

Figure 5

CREB3L4 significantly decreased the chemosensitivity of HCC cells to sorafenib by up-regulating RHEB-mTORC1 axis

HUH7 and Hep3B cells were transfected with shCREB3L4 and HA-CREB3L4.

(A and B) Cells were treated with different dosage (0, 0.01 μM, 0.1 μM, 1 μM, 10 μM, and 100 μM) of sorafenib for 48 h, then CCK-8 assay was performed to detect cells viabilities.

(C and D) Cells were treated with sorafenib (10 μM) for 48 h, then CCK-8 assay was performed to detect cells viability.

(E and F) Colony formation assay was performed to detect the proliferation of the cells treated with sorafenib (10 μM).

(G and H) HUH7 and Hep3B cells were transfected with CREB3L4 or shCREB3L4. Approximately 48 h after transfection, the expression levels of mTOR signaling associated proteins including RHEB, p-mTOR, mTOR, p-S6K1, S6K1, p-S6, S6, p-4E-BP1, and 4E-BP1 were detected. ∗∗p < 0.01, ∗∗∗p < 0.001 for statistical analysis of the indicated groups.