Figure 4.

The ECM is differentially distributed within musculoskeletal tissues during forelimb development

(A–R) Cryosections from E11.5-E14.5, P3 and adult forelimbs were stained with antibodies against: (A–F, A′–D′) type I collagen (COLI; green), type V collagen (COLV; red), and myosin heavy chain, a marker for differentiated skeletal muscle (MY32; blue); (G–L, G′–J′) tenascin-C (TNC; green), fibrillin-2 (FBN2; red), and MY32 (blue); (M–R, M′–P′) elastin microfibril interfacer-1 (EMILIN1; green), perlecan (HSPG2; red), and nidogen-2 (NID2; blue). Insets (indicated with ’) are a 3× enlargement of the region containing the nascent elbow (∗) for E11.5-E14.5. Scale bars represent 200 μm. Individual channels and secondary antibody only negative control panels are shown in Figures S3–S5.

(S) Graphical summary of protein dynamics.