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. 2024 Jan 10;4(1):100684. doi: 10.1016/j.crmeth.2023.100684

Figure 2.

Figure 2

AAV.3x(core)mscRE4.YFP labels dentate granule cells in dorsal DG

(A) AAV.3x(core)mscRE4.YFP and AAV.CaMKIIα.mCherry were unilaterally injected together in the dorsal DG in one hemisphere of young adult (7–11 weeks old) wild-type mice (n = 5; 3 females and 2 males) and expressed for 1–2 weeks prior to acute slice preparation.

(B and C) YFP/mCherry co-expression appeared selective for granule cells in confocal images (40× objective; oil) of “magic cut” slices (B), which was further assessed by (C) cell counting in the DG-hilus region, where mCherry+ hilar cells were consistently YFP−, and 77.2% mCherry+ cells in the granule cell layer were YFP+.

(D–G) Line scan profiles in the DG (D and E) in the injected hemisphere and (F and G) in the non-injected hemisphere depicting the relative brightness of DAPI, YFP, and mCherry signals across a normalized distance from the hilus. Scale bars: 100 μm throughout.