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AAV.3x(core)mscRE4.YFP expression in mossy fibers but not CA3 pyramidal neurons
(A) Co-injection of AAV.3x(core)mscRE4.YFP and AAV.CaMKIIα.mCherry into the dorsal DG in one hemisphere of adult (7–11 weeks old) wild-type mice produced (B) mCherry expression in CA3 somas along with a lack of YFP expression in CA3 somas, as well as (C1–C3) co-expression of mCherry and YFP in mossy fiber axons projecting from the DG (as seen in confocal images; 40× objective, oil). Scale bars: 50 μm.
