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. 2024 Jan 10;4(1):100684. doi: 10.1016/j.crmeth.2023.100684

Figure 4.

Figure 4

AAV.3x(core)mscRE4.YFP and AAV.CaMKIIα.mCherry labeling in ventral hippocampus

(A) Adult wild-type mice were co-injected with AAV.3x(core)mscRE4.YFP and AAV.CaMKIIα.mCherry in the ventral DG (vDG).

(B) After 1–2 weeks, in confocal images (20× objective; horizontal sections), we observed mCherry labeling in DG granule cell somas, as well as putative hilar mossy cells and CA3 pyramidal cells.

(C and D) We also observed (C) YFP labeling in a subset of DG granule cells somas, with a noticeable absence of somatic labeling in the hilus or CA3, and thus (D) co-expression of mCherry and YFP labeling was restricted to the DG granular layer. Scale bars: 100 μm.