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. 2024 Jan 10;4(1):100684. doi: 10.1016/j.crmeth.2023.100684

Figure 5.

Figure 5

Genetic manipulation of DG granule cells using AAV.mscRE4.Cre

(A) A schematic of the experimental layout to achieve genetic manipulation of DG granule cells through retro-orbital (RO) injections of AAV.mscRE4.Cre, followed later by stereotaxic injection of the floxed construct AAV.hSyn.DIO.mCherry.

(B) Sparse mCherry labeling in the DG granular layer somas and in CA3 observed 2–3 weeks after stereotaxic injection in confocal images (10× objective).

(C) Higher-magnification images (60× objective) in CA3 revealed sparse red axonal labeling.

(D1–D3) Stereotaxic co-injection of two floxed constructs, AAV.EF1α.DIO.mitoGFP and AAV.hSyn.DIO.mCherry, produced mitochondrial labeling in DG granule cells, including their mossy fiber boutons (MFBs).

Scale bars: 100 μm (B) and 10 μm (C and D1–D3).