Figure 6.

Ability of MnO₂@TMNP to specifically target macrophages for ROS clearance. (A) Flowchart for the evaluation of macrophages taking up nanoparticles. (B) Flow cytometry results of the uptake of DiO-loaded nanoparticles by different cells. (C) Statistical analysis of the positive DiO rate of different cells. (D) Fluorescence images of the fusion of DiD-loaded nanoparticles and DiO-loaded macrophages. Scale bar: 20 μm. (E) Records of dissolved oxygen in water to assess the H₂O₂ decomposition catalyzed by MnO₂@TMNP, MnO₂ nanoparticles, or TMNP. (F) Flow cytometry of intracellular ROS indicating the effects of MnO₂@TMNP, MnO₂ nanoparticles, or TMNP on scavenging ROS. The ROS intensity was normalized to the control group. Data are presented as the mean ± SD (n = 3): ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001 between groups.