TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| M. catarrhalis | ||
| 035E | Wild-type isolate from middle ear fluid | 27 |
| 035E.1 | Isogenic mutant of 035E with a kan cartridge in the uspA1 structural gene | 1 |
| 035E.2 | Isogenic mutant of 035E with a kan cartridge in the uspA2 structural gene | 1 |
| 035E.12 | Isogenic mutant of 035E with a kan cartridge in the uspA2 structural gene and a cat cartridge in the uspA1 structural gene | This study |
| P-44 | Wild-type isolate that exhibits rapid hemagglutination | 52 |
| P-48 | Wild-type isolate that exhibits slow hemagglutination | 52 |
| Escherichia coli DH5α | Host for cloning experiments | Stratagene |
| Plasmids | ||
| pBluescript II SK+ | Cloning vector; Ampr | Stratagene |
| pUSPA1 | pBluescript II SK+ with a 2.7-kb insert containing most of the uspA1 gene of M. catarrhalis 035E | 1 |
| pUSPA1CAT | pUSPA1 with a cat cartridge replacing the 0.6-kb BglII fragment of the uspA1 gene | This study |