TABLE 2.
Adherence of wild-type and mutant strains of M. catarrhalis to HEp-2 and Chang conjunctival cells in vitro
| Strain | Adherencea to:
|
|
|---|---|---|
| HEp-2 cellsb | Chang cellsc | |
| 035E (wild type) | 14.7 ± 4.9 | 51.4 ± 30.8 |
| 035E.1 (uspA1 mutant) | 2.4 ± 0.9 (0.006d) | 0.8 ± 0.5 (0.002) |
| 035E.2 (uspA2 mutant) | 19.1 ± 7.0 (0.213) | 55.9 ± 16.7 (0.728) |
| 035E.12 (uspA1 uspA2 double mutant) | 2.3 ± 1.8 (0.011) | 0.6 ± 0.2 (0.002) |
a
Expressed as percentage of the original inoculum that was adherent to the human epithelial cells at the end of the 30-min incubation period. Each value represents the mean ± standard deviation of two independent experiments.
b
PBS was used for washing of the monolayers and for serial dilutions of adherent M. catarrhalis.
c
PBS-G was used for washing of the monolayers and for serial dilutions of adherent M. catarrhalis.
d
P value compared to wild-type strain 035E, using the two-tailed Student t test.