TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Reference or source |
|---|---|---|
| V. vulnificus strains | ||
| CML17 | 80363 Δ(fur) | 28 |
| MO6-24 | Polyr, opaque | 55 |
| CML49 | MO6-24, Δ(hupA) | This study |
| E. coli strains | ||
| DH5α | F−endA1 hsdR17 supE44 thi-1 recA1 gyrA96 relA1 Δ(argF-lacZYA)U169 (φ80ΔlacZ M15)λ− | 18 |
| ABLE K | lac(LacZ−) [Kanr McrA− McrCB− McrA− McrF− Mrr− HsdR (rK− mK−)] [F′ proAB lacIqZΔ M15 Tn10 (Tetr)] | Stratagene |
| SY327λpir | Δ(lac pro) nalA recA56 araD argE(Am) λpir R6K | 30 |
| SM10λpir | thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu λpir R6K Kmr | 30 |
| Plasmids | ||
| pUC19 | Cloning vector; Apr | Laboratory stock |
| pBluescript SK− | Phagemid derived from pUC19; Apr | Stratagene |
| pLAFR3 | Cloning vector; Tcr | 48 |
| pCML37 | V. vulnificus hupA clone from V. vulnificus MO6-24 genomic library; 8-kbp chromosomal fragment in pBluescript SK−; Apr | This study |
| pCML38 | 1.7-kbp HindIII V. vulnificus hupA clone in pBluescript SK−; Apr | This study |
| pCML40 | pBluescript SK− with HindIII insertion of V. vulnificus hupA, containing an internal 567-bp BglII-EspI deletion; Apr | This study |
| pCVD442 | Positive selection suicide vector, pGP704 with sacB gene inserted in multiple cloning site; Apr | 10 |
| pCML41 | pCVD442 with HindIII insertion of V. vulnificus hupA, containing an internal 567-bp BglII-EspI deletion; Apr | This study |
| pCML42 | 2.3-kbp NcoI-BamHI hupA fragment and pBluescript cloned into the BamHI site of pLAFR3; Tcr Apr | This study |
a
Apr, ampicillin resistance; Kmr, kanamycin resistance; Polyr, polymyxin B resistance; Tcr, tetracycline resistance.