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. 2024 Jan 22;41:101860. doi: 10.1016/j.tranon.2023.101860

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© 2023 The Authors. Published by Elsevier Inc.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig 2 — Activation of partial EMT in nutrient deficient Caco-2 cells. (A) qRT-PCR of EMT genes in NR and ND treated CRC cells. (B) EMT Score of ND CRC cell lines. (C) STRING analysis of significantly (p<0.01, LFC ±1) DE genes related to EMT. (D) Representative images of IF staining for α-Tubulin and LAMP1 in NR and ND Caco-2 cells. (E) Subcellular localization analysis of LAMP1 signal. Nuclear (1), perinuclear (2), and peripheral (3) regions. (F) Analysis of lysosomal abundance. Data are represented as mean ± SEM (*p < 0.05, **p < 0.01, ***p < 0.001 with respect to NR).