Fig 4.

Involvement of the cytoskeletal network in the induction of partial EMT with nutrient deficiency. (A) qRT-PCR of CDH1 and VIM in Colc+BafA1 (5 nM) treated ND Caco-2 cells. Data are presented as mean ± SD. (B) Representative images of IF staining for α-Tubulin and LAMP1 in ND+BafA1 (5 nM) treated Caco-2 cells with or without Colchicine (Colc). Zoomed images are shown in the right panel. White arrow heads indicate the lysosomes in protrusions. (C) qRT-PCR of MCOLN1 in nutrient-restricted and BafA1 (100 nM)-treated Caco-2 cells. Data are presented as mean ± SEM. (*p < 0.05, **p < 0.01, ***p < 0.001, t-test).