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. 2024 Jan 11;17(1):e14400. doi: 10.1111/1751-7915.14400

FIGURE 1.

FIGURE 1

Design of synthetic microbial consortia with P. putida and C. glutamicum. (A) Monoculture of P. putida KT2440 (WT) and C. glutamicum ATCC13032 (WT) with the fluorescence proteins Crimson and GfpUV indicated with the colours red and green, respectively. (B) Co‐culture with P. putida WT and C. glutamicum WT without any interaction (0/0) growing in the same medium. (C) Co‐culture of P. putida KT2440 ΔargH as arginine‐auxotrophic strain with the arginine‐overproducer C. glutamicum ARG2 in a commensal consortium (+/0) via arginine dependency of P. putida. (D) Co‐culture of P. putida FORM as formamide‐utilising strain with formamidase‐negative C. glutamicum WT in a commensal consortium (0/+) via ammonium dependency of C. glutamicum. (E) Co‐culture of the arginine‐auxotrophic, formamide‐utilising strain P. putida KT2440 ΔargH FORM with the arginine‐overproducer strain C. glutamicum ARG2, which cannot utilise formamide, in a cooperative consortium (+/+) via arginine and ammonium interdependency.