Fig 6.

Cytokine mRNA expression in PBMC from normal cats and cats with rhinitis. PBMC were isolated by density centrifugation from blood of group 2 cats (control, n=8), group 3 cats (client, n=9), and group 4 cats (shelter, n=28), prior to treatment based on sample availability. Total RNA was isolated from cells immediately after isolation (A) or following stimulation for 6 h with phorbol myristate acetate (PMA: 10 ng/ml) plus ionomycin (500 ng/ml) (B) and cytokine mRNA expression was quantitated as described in Materials and methods. Mean cytokine expression levels (±SD) were expressed relative to a sample of pooled cDNA and plotted. ∗ Denotes values that are statistically different (P<0.05) from the control group.