FIG. 2.

Stability of peptide binding to MHC-I 2D^b molecules. RMA-S cells were incubated at 25°C with various concentrations of peptides and then transferred to 37°C and incubated for the indicated times. The cells were then washed at 4°C and stained as described in the legend to Fig. 1. The results are expressed as mean fluorescence of duplicate determinations ± standard deviation from one of two representative experiments (P1 [A], P3 [B], and P4 [C]).