FIG. 3.

Stability of lipopeptide binding to MHC-I 2D^b molecules. RMA-S cells were incubated at 25°C with various concentrations of lipopeptides and then transferred to 37°C and incubated for the indicated times. The cells were then washed at 4°C and stained as described in the legend to Fig. 1. The results are expressed as mean fluorescence of duplicate determinations ± standard deviation from one of two representative experiments (LP1 [A], LP3 [B], and LP4 [C]).